polyclonal rabbit anti scarb1  (Proteintech)

Journal: Biomedical research (Tokyo, Japan)

Article Title: A role for scavenger receptor B1 as a captor of specific fatty acids in taste buds of circumvallate papillae.

doi: 10.2220/biomedres.39.295

Figure Lengend Snippet: Fig. 1 Assessment of the expression and distribution of SR-B1 in mouse circumvallate papillae. (A) RT-PCR analysis. Total RNA samples from circumvallate papillae (CP) and liver (L) were analysed. PCR reactions were conducted with (RT (+)) or without (RT (−)) reverse transcriptase. A 706 bp band was seen in each of the lanes for reaction with transcriptase. Lane M shows the migration pattern of a DNA size marker (100 bp DNA Ladder; New England Biolabs Japan, Tokyo) through the gel. The positions where 100-, 500-, and 1000-bp fragments migrate are indicated on the left in base pairs (bp). (B) Immu- nohistochemical staining analysis. A section of tongue containing circumvallate papillae was probed with an anti-SR-B1 an- tibody (NB400-104). SR-B1-immunopositive cells in which the apical part was preferentially labelled were indicated by arrowheads. Bar: 50 μm. (C) Double immunostaining of a section from circumvallate papillae with another anti-SR-B1 anti- body (TA301489) (top) and an anti-CD36 antibody (AF2519) (middle). The merged image is shown in the bottom panel. The majority of CD36-immunoreactive cells were SR-B1-positive. Bar in the top panel: 50 μm.

Article Snippet: For double immunofluorescence staining, the sections were incubated overnight with another rabbit anti-SR-B1 antibody (TA301489; OriGene, Rockville MD, USA) (1 : 200) and a goat anti-CD36 antibody (AF2519; R&D Systems, Minnesota, MN, USA) (1 : 200).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Reverse Transcription, Migration, Marker, Staining, Double Immunostaining

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: The sections stained with isotype matched normal rabbit IgG served as negative control (the control images of ABCA1 and SR-BI were in the or ). ( A ) Images stained with ABCA1 and SR-BI antibodies on Days 12, 15 and 18 of pregnency in Yorkshire pigs. The results show that the ABCA1 signals were observed mainly to LE at the mesometrial side and were barely detected in LE at the anti-mesometrial side. The positive signals were also barely detected in Tr and GE. The SR-BI signals were observed in LE, GE and Tr. The stain intensity of SR-BI in LE between at the mesometrial side and the anti-mesometrial side was similar. ( B ) Quantitative analysis of ABCA1 and SR-BI by measuring the average integrated optical density (IOD) in LE in Yorkshire pigs. Asterisks indicate significant differences (mean ± SD) between breeds (**P < 0.01), P value was determined by Student’s t test. Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; D, day of pregnancy; Scale bars = 100 μm or 20 μm (amplification).

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Staining, Negative Control, Amplification

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: ( A ) Images stained with ABCA1 and SR-BI antibodies on Days 12, 15 and 18 of pregnency in Meishan pigs. The expression patterns of ABCA1 and SR-BI both in Meishan pigs and Yorkshire pigs were similar. ( B ) Quantitative analysis of ABCA1 and SR-BI by measuring the average integrated optical density (IOD) in LE. Asterisks indicate significant differences (mean ± SD) between breeds (**P < 0.01), P value was determined by Student’s t test. Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; D, day of pregnancy; Scale bars = 100 μm or 20 μm (amplification).

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Staining, Expressing, Amplification

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: Positive signals of ABCA1 and SR-BI were shown in red and the blue staining represented nuclei (DAPI stained). The signals of ABCA1 and SR-BI were principally localized at the basolateral and apical side of the endometrial LE cell membrane. The sections stained with isotype matched normal rabbit IgG served as negative control (the control images of ABCA1 and SR-BI were in the or ). Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; D, day of pregnancy; Scale bar = 40 μm.

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Staining, Negative Control

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: Uterine/placental sections were obtained on Days 26, 50 and 95 of pregnancy. Tissue sections were stained with rabbit anti-human ABCA1 polyclonal antibody.The sections stained with isotype matched normal rabbit IgG served as negative control. ( A ) Images were taken from the maternal-fetal interface in Yorkshire and Meishan pigs. ABCA1 positive cells were observed in the epithelial bilayer and GE in Yorkshire and Meishan pigs on Days 26, 50 and 95 of pregnancy. Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; D, day of pregnancy; NC, negative control; Scale bar = 100 μm. ( B ) Quantitative analysis of ABCA1 by measuring the average integrated optical density (IOD) in the epithelial bilayer (composed by Tr and LE) and GE during pregnancy. Asterisks indicate significant differences (mean ± SD) between breeds (*P < 0.05; **P < 0.01. Analyzed by PROC MIXED of SAS). ( C ) Images were taken from the placental areolae in Yorkshire and Meishan pigs. The ABCA1 positive signals were filled in all the areolar regions, including Tr, LE and GE on Days 26, 50 and 95 of pregnancy. AR, areola; Scale bar = 100 μm.

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Staining, Negative Control

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: Uterine/placental sections were obtained on Days 26, 50 and 95 of pregnancy. Tissue sections were stained with rabbit anti-human SR-BI polyclonal antibody. The sections stained with isotype matched normal rabbit IgG served as negative control. ( A ) Images were taken from the maternal-fetal interface in Yorkshire and Meishan pigs. SR-BI positive cells were observed in Tr, LE and GE in Yorkshire and Meishan pigs on Days 26, 50 and 95 of pregnancy. Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; D, day of pregnancy; NC, negative control; Scale bar = 100 μm. ( B ) Quantitative analysis of SR-BI by measuring the average integrated optical density (IOD) in the epithelial bilayer (composed by Tr and LE) and GE during pregnancy. Asterisks indicate significant differences (mean ± SD) between breeds within a day (**P < 0.01. Analyzed by PROC MIXED of SAS). The data were shown as the mean ± SD. ( C ) Images were taken from the placental areolae in Yorkshire and Meishan pigs. The SR-BI positive signals were filled with all the areolar regions, including Tr, LE and GE on Days 26, 50 and 95 of pregnancy. AR, areola ; Scale bar = 100 μm.

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Staining, Negative Control

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: Positive staining of SR-BI is shown in red, blue staining represents nuclei (DAPI stained). The signals were mostly diffused in cells of Tr, LE and GE in Yorkshire and Meishan pigs on Days 26, 50 and 95 of pregnancy. And specially on Day 95 of pregnancy, in Yorkshire pigs, SR-BI staining were mostly observed in Tr and just few positive signals were observed in LE at the maternal-fetal interface; while in Meishan pigs, SR-BI signals were strongly located at the maternal-fetal interface, including all the Tr and LE. Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; D, day of pregnancy; NC, negative control; Scale bar = 40 μm.

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Staining, Negative Control

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: The sections stained with isotype matched normal rabbit IgG served as negative control (the control images of ABCA1 and SR-BI were in the or ). ( A ) Images stained with ABCA1 and SR-BI antibodies on Days 12, 15 and 18 of pregnency in Yorkshire pigs. The results show that the ABCA1 signals were observed mainly to LE at the mesometrial side and were barely detected in LE at the anti-mesometrial side. The positive signals were also barely detected in Tr and GE. The SR-BI signals were observed in LE, GE and Tr. The stain intensity of SR-BI in LE between at the mesometrial side and the anti-mesometrial side was similar. ( B ) Quantitative analysis of ABCA1 and SR-BI by measuring the average integrated optical density (IOD) in LE in Yorkshire pigs. Asterisks indicate significant differences (mean ± SD) between breeds (**P < 0.01), P value was determined by Student’s t test. Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; D, day of pregnancy; Scale bars = 100 μm or 20 μm (amplification).

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Staining, Negative Control, Amplification

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: ( A ) Images stained with ABCA1 and SR-BI antibodies on Days 12, 15 and 18 of pregnency in Meishan pigs. The expression patterns of ABCA1 and SR-BI both in Meishan pigs and Yorkshire pigs were similar. ( B ) Quantitative analysis of ABCA1 and SR-BI by measuring the average integrated optical density (IOD) in LE. Asterisks indicate significant differences (mean ± SD) between breeds (**P < 0.01), P value was determined by Student’s t test. Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; D, day of pregnancy; Scale bars = 100 μm or 20 μm (amplification).

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Staining, Expressing, Amplification

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: Positive signals of ABCA1 and SR-BI were shown in red and the blue staining represented nuclei (DAPI stained). The signals of ABCA1 and SR-BI were principally localized at the basolateral and apical side of the endometrial LE cell membrane. The sections stained with isotype matched normal rabbit IgG served as negative control (the control images of ABCA1 and SR-BI were in the or ). Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; D, day of pregnancy; Scale bar = 40 μm.

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Staining, Negative Control

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: Uterine/placental sections were obtained on Days 26, 50 and 95 of pregnancy. Tissue sections were stained with rabbit anti-human ABCA1 polyclonal antibody.The sections stained with isotype matched normal rabbit IgG served as negative control. ( A ) Images were taken from the maternal-fetal interface in Yorkshire and Meishan pigs. ABCA1 positive cells were observed in the epithelial bilayer and GE in Yorkshire and Meishan pigs on Days 26, 50 and 95 of pregnancy. Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; D, day of pregnancy; NC, negative control; Scale bar = 100 μm. ( B ) Quantitative analysis of ABCA1 by measuring the average integrated optical density (IOD) in the epithelial bilayer (composed by Tr and LE) and GE during pregnancy. Asterisks indicate significant differences (mean ± SD) between breeds (*P < 0.05; **P < 0.01. Analyzed by PROC MIXED of SAS). ( C ) Images were taken from the placental areolae in Yorkshire and Meishan pigs. The ABCA1 positive signals were filled in all the areolar regions, including Tr, LE and GE on Days 26, 50 and 95 of pregnancy. AR, areola; Scale bar = 100 μm.

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Staining, Negative Control

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: Positive staining of ABCA1 was shown in red and the staining was diffused in the positive cells (except the nuclei) without specific subcellular localization. The blue staining represents nuclei (DAPI stained). In Yorkshire pigs, the ABCA1 staining were mostly observed in LE on Days 26 and 50 of pregnancy, while on Day 95 of pregnancy, the positive signals were mostly observed in Tr and just a few positive signals could be observed in LE. In Meishan pigs, the ABCA1 staining was observed both in Tr and LE on Days 26, 50 and 95 of pregnancy. Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; D, day of pregnancy; NC, negative control; Scale bar = 40 μm.

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Staining, Negative Control

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: ( A ) schematic drawing of the epitheliochorial placenta of pig. ( B ) the maternal-fetal interface of the pig placenta composed of two regions, the interareolar region (composed by trophoblast and luminal epithelium, outside of arrowheads) and the areolar region (located at the mouth of uterine glands, enclosed by arrowheads), respectively. Our findings revealed two paths of cholesterol transport from maternal to fetal circulation, 1 the areolar-gland subunit; 2 the placental epithelial bilayer; ( C ) Schematic illustration of ABCA1 expression patterns in placental trophoblast in Yorkshire and Meishan pigs, showing that the ABCA1 (in red) was barely expressed in trophoblast which located at the interareolar region (outside of arrowheads) in Yorkshire, but it was highly expressed in the trophoblast in Meishan pigs. ( D ) Schematic illustration of the proposed mechanism for Meishan pig to increase the placental efficiency in transport cholesterol. Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; AR, areola of placenta.

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Expressing